Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney.
نویسندگان
چکیده
A novel location of the bile-acid-conjugating enzyme bile acid-CoA:amino acid N-acyltransferase (BAT) has been discovered in the cytosolic fraction of rat kidney. Both taurine and glycine were utilized as substrates. Formation of bile acid N-acyl amidates was verified by h.p.l.c. by comparison with authentic standards and by specific hydrolysis using cholylglycine hydrolase. Immunoblot analysis using a human liver anti-BAT polyclonal antibody indicated that rat kidney BAT has the same molecular mass as rat liver BAT. These findings suggest that the kidney has a role in bile acid metabolism and physiology.
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عنوان ژورنال:
- The Biochemical journal
دوره 280 ( Pt 3) شماره
صفحات -
تاریخ انتشار 1991